Contact:

Laboratory of Separation and Spectroscopic Method Application
Institute of Biological Sciences

Faculty  of Medicine
The John Paul II Catholic University of Lublin
Konstantynow 1J, 20-708 Lublin, Polan

ilona.sadok@kul.pl

https://www.researchgate.net/profile/Ilona_Sadok

https://orcid.org/0000-0003-1154-7581

 

For more information on the issue, please visit the Special Issue website at
https://www.mdpi.com/journal/sensors/special_issues/Electrochem_Sens_Determ_Bio

 

https://www.mdpi.com/journal/sensors/topical_collections/S2C7S3JZ7N

 

https://www.mdpi.com/journal/sensors/special_issues/74DYX1MV1T

 

I am interested in the development of novel approaches towards the measurement of biologically important molecules that might play key roles in human disease pathogenesis. Currently, I am focused on tryptophan metabolites via the kynurenine pathway (named kynurenines). I am developing analytical tools (utilizing liquid chromatography, mass spectrometry, voltammetry) for monitoring of kynurenines concentrations in samples from patients to understand how changes in the regulation of tryptophan metabolism impact the mechanisms of human diseases (like cancer).

Projects in progress:

Application of LC and mass spectrometry for monitoring of tryptophan metabolites (kynurenines) in disease states

Goal: Development of fully validated analytical protocols that serve for tryptophan and its metabolites via kynurenine pathway monitoring in a variety of biological samples: cancer cell cultures, sera, and other material collected from cancer patients or from patients with pregnancy problems. The project assumes the application of LC- MS or LC-MS/MS for analytes quantification (details).

Development of voltammetric protocols for tryptophan metabolites (kynurenines) determination

Goal: Study the voltammetric behavior of tryptophan and kynurenines at bare and chemically modified electrodes. Development of simple and rapid voltammetric methods for determination of kynureniens in a variety of biological milieus (details).

The role of glycation and kynurenine pathway in the modulation of cancer environment

Goal: Study kynurenine pathway activation in presence of different protein glycation products. Application among other HPLC-DAD for IDO activity estimation, LC-MS for kynurenines quantification in cancer-origin samples.

Mycotoxin analysis in food

Goal: Application of LC-MS/MS, HPLC-DAD for mycotoxin analysis in a variety of fruit-based products. The project is mainly focused on difficult to quantification mycotoxins (like patulin) and will provide new strategies for sample pre-treatment and chromatographic analysis to achieve detection levels meeting legislative levels (details).

Other interest and skills:

Functionalized polysiloxanes as electrode surface modifiers

Goal: Development of voltammetric sensors for metal ions determination by simultaneous modification of the electrode surface with thiol-functionalized polysiloxane film and metal particles (details).

Metal particles Nafion covered electrodes in voltammetric assays

Goal: Utilization of strategy of simultaneous modification of the electrode surface with the Nafion film and electrodeposited metal particles (Pb, Bi) for development of new voltammetric sensors for determinations of biologically active compounds in real samples (details).

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